Fine Mapping of Two Additive Effect Genes for Awn Development in Rice (Oryza sativa L.)
نویسندگان
چکیده
Awns, important domestication and agronomic traits in rice (Oryza sativa L.), are conferred by polygenes and the environment. Near isogenic line (NIL) pairs BM33 and BM38 were constructed from crosses between awnless japonica cv Nipponbare as recurrent parent, and lines SLG or Funingxiaohongmang (awned japonica accessions), respectively, as donors. In order to study the genetic and molecular mechanism of awning, two unknown, independent genes with additive effects were identified in a cross between the NILs. To map and clone the two genes, a BC4F4 population of 8,103 individuals and a BC4F6 population of 11,206 individuals were constructed. Awn3-1 was fine mapped to a 101.13 kb genomic region between Indel marker In316 and SNP marker S9-1 on chromosome 3. Nine predicted genes in the interval were annotated in the Rice Annotation Project Database (RAP-DB), and Os03g0418600 was identified as the most likely candidate for Awn3-1 through sequence comparisons and RT-PCR assays. Awn4-2 was fine mapped to a 62.4 kb genomic region flanked by simple sequence repeat (SSR) marker M1126 and Indel maker In73 on chromosome 4L. This region contained the previously reported gene An-1 that regulates awn development. Thus, An-1 may be the candidate gene of Awn4-2. These results will facilitate cloning of the awn genes and thereby provide an understanding of the molecular basis of awn development.
منابع مشابه
Identification of major and minor genes associated with heading date in an indica × indica cross of rice (Oryza Sativa L.)
In this study, quantitative trait loci (QTLs) controlling rice heading date were detected in a F2:3 population derived from a cross between an indica rice variety, Tarom Mahalli, with early heading date, and an indica variety, Khazar, with late heading date. SSR linkage map was constructed using 74 polymorphic markers and 192 F2 individuals and covered a total of 1231.50 cM of rice genome. QTL ...
متن کاملConvergent Loss of Awn in Two Cultivated Rice Species Oryza sativa and Oryza glaberrima Is Caused by Mutations in Different Loci
A long awn is one of the distinct morphological features of wild rice species. This organ is thought to aid in seed dispersal and prevent predation by animals. Most cultivated varieties of Oryza sativa and Oryza glaberrima, however, have lost the ability to form long awns. The causal genetic factors responsible for the loss of awn in these two rice species remain largely unknown. Here, we evalu...
متن کاملIdentification of main and epistatic QTLs for grain yield related traits in a recombinant inbred lines population of rice (Oryza sativa L.)
Rice yield as a complex trait is the main target in most rice breeding programs. To map the main and epistatic QTLs controlling grain yield and yield components, an experiment was carried out using a 129 F6 recombinant inbred lines population (IRA population) originated from a cross between Alikazemi / IR67017-180-2-1-2, in 2015 growing season in two locations, Rasht and Tonekabon, Iran. The e...
متن کاملComparative Mapping of Seed Dormancy Loci Between Tropical and Temperate Ecotypes of Weedy Rice (Oryza sativa L.)
Genotypic variation at multiple loci for seed dormancy (SD) contributes to plant adaptation to diverse ecosystems. Weedy rice (Oryza sativa) was used as a model to address the similarity of SD genes between distinct ecotypes. A total of 12 quantitative trait loci (QTL) for SD were identified in one primary and two advanced backcross (BC) populations derived from a temperate ecotype of weedy ric...
متن کاملارزیابی ژنتیکی صفات کمی برنج (Oryza sativa L.) با استفاده از روش تجزیه میانگین نسلها
In order to study mode of gene action in rice for the traits related to yield, four varieties of rice were investigated. Direct and reciprocal crosses have been done between parents Sang-Tarrom and Gerdeh, IR229 and IRRI2 and their back crosses. The F1 and BC seeds were grown in 2006 and F1 plants were selfed for obtaining F2 seeds. Therefore, 10 different populations (generations) including ...
متن کامل